Microscope and photo stacking

Introduction

Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods.

Theory

We use Raspberry Pi 4 or Zero with 12 MP camera. The camera supports C and CS mount lenses (CGL 6 mm CS-mount and 16 mm C-mount lenses). Also M/CS mount adapter is available.

Screen

The small screen is a 3.5 inches TFT LCD capacitive touchscreen with a 480×320 resolution that supports up to 1920×1080. It is connected to the Raspberry Pi with a HDMI adapter for display, and to the GPIO port for the touchscreen function.

Magnification

Aim: Wide magnification range: Samples with features from several centimeters to several micrometers can be imaged without changing the objective lens.

• Pimoroni Microscope Lens 60x-900x https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi

Illumination

Reflected light

Transmitted light

Cross-section or contact angle

References

A lot of different ideas. Some are listed below

• https://www.raspberrypi.org/blog/a-low-cost-open-source-computer-assisted-microscope/
• https://microscopiproject.wordpress.com/
• https://www.raspberrypi.org/blog/raspberry-pi-high-quality-camera-powers-up-homemade-microscope/ Excellent images https://github.com/IBM/MicroscoPy
• https://hackaday.io/project/167996-pi-microscope
• https://www.instructables.com/ARPM-Another-raspberry-pi-microscope-made-from-Ple/
• https://publiclab.org/notes/MaggPi/04-08-2018/raspberry-pi-microscope-close-up-lens-system
• https://www.briandorey.com/post/raspberry-pi-high-quality-camera-on-the-microscope
• https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi
• https://openlabwaredotnet.files.wordpress.com/2015/11/main-v4.pdf
• https://magpi.raspberrypi.org/articles/openflexure-microscope
• https://diyprojects.io/motorized-microscope-hq-camera-raspberry-pi-python-html-interface/#.YRPo7YgzZEY