Microscope and photo stacking: Difference between revisions
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Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods. | Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods. | ||
https://web.archive.org/web/20160917212243/http://photoblog.edu-perez.com/2009/01/greater-depth-field-macro.html | |||
== Theory == | == Theory == | ||
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We use Raspberry Pi 4 or Zero with [https://www.raspberrypi.org/products/raspberry-pi-high-quality-camera/ 12 MP camera]. The camera supports C and CS mount lenses (CGL 6 mm CS-mount and 16 mm C-mount lenses). Also M/CS mount adapter is available. | We use Raspberry Pi 4 or Zero with [https://www.raspberrypi.org/products/raspberry-pi-high-quality-camera/ 12 MP camera]. The camera supports C and CS mount lenses (CGL 6 mm CS-mount and 16 mm C-mount lenses). Also M/CS mount adapter is available. | ||
=== Magnification === | === Screen === | ||
The small screen is a [[3.5 inches TFT LCD capacitive touchscreen raspberry pi|3.5 inches TFT LCD capacitive touchscreen]] with a 480×320 resolution that supports up to 1920×1080. It is connected to the Raspberry Pi with a HDMI adapter for display, and to the GPIO port for the touchscreen function. | |||
=== Lenses and Magnification === | |||
HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field. | |||
The ocular is a magnifier meant to look at the intermediate image formed by the objective lens. Eye looks into eyepiece, thus it should produce a virtual image at infinity (the eye is relaxed). The object plane of the ocular is the image plane of object. | |||
Total magnification: <math>M = M_\text{objective} \times M_\text{ocular}</math>. | |||
Aim: Wide magnification range: Samples with features from several centimeters to several micrometers can be imaged without changing the objective lens. | |||
Particles with diameters between 1um and 10um. https://publiclab.org/notes/partsandcrafts/02-15-2018/2-attaching-your-raspberry-pi-camera-to-a-microscope-objective-lens | |||
Microscope lenses: RMS Threaded stands for ''Royal Microscopy Society''. | |||
https://www.seeedstudio.com/Microscope-Camera-300X-C-Mount-Lens-for-Raspberry-Pi-High-Quality-Camera-p-4627.html | |||
https://www.amscope.com/accessories/objective/100x-oil-achromatic-microscope-objective-for-compound-microscopes.html | |||
https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/ | |||
https://www.cambridgeincolour.com/tutorials/macro-lenses.htm | |||
* Pimoroni Microscope Lens 60x-900x https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi | |||
=== Motor === | === Motor === | ||
=== Stacking Photos === | === Stacking Photos === | ||
Aligning: http://hugin.sourceforge.net/ | |||
http://enblend.sourceforge.net/index.htm http://enblend.sourceforge.net/enfuse.doc/enfuse_4.1.xhtml/enfuse.xhtml#Focus-Stacks | |||
https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/ | |||
https://www.cambridgeincolour.com/tutorials/focus-stacking.htm | |||
=== Illumination === | |||
Reflected light | |||
Transmitted light | |||
Cross-section or contact angle | |||
== References == | |||
A lot of different ideas. Some are listed below | |||
* https://www.raspberrypi.org/blog/a-low-cost-open-source-computer-assisted-microscope/ | |||
* https://microscopiproject.wordpress.com/ | |||
* https://www.raspberrypi.org/blog/raspberry-pi-high-quality-camera-powers-up-homemade-microscope/ Excellent images https://github.com/IBM/MicroscoPy | |||
* https://hackaday.io/project/167996-pi-microscope | |||
* https://www.instructables.com/ARPM-Another-raspberry-pi-microscope-made-from-Ple/ | |||
* https://publiclab.org/notes/MaggPi/04-08-2018/raspberry-pi-microscope-close-up-lens-system | |||
* https://www.briandorey.com/post/raspberry-pi-high-quality-camera-on-the-microscope | |||
* https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi | |||
* https://openlabwaredotnet.files.wordpress.com/2015/11/main-v4.pdf | |||
* https://magpi.raspberrypi.org/articles/openflexure-microscope | |||
* https://diyprojects.io/motorized-microscope-hq-camera-raspberry-pi-python-html-interface/#.YRPo7YgzZEY | |||
* https://publiclab.org/notes/partsandcrafts/11-26-2017/building-a-raspberry-pi-microscope | |||
Latest revision as of 15:29, 12 August 2021
Introduction
Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods.
Theory
We use Raspberry Pi 4 or Zero with 12 MP camera. The camera supports C and CS mount lenses (CGL 6 mm CS-mount and 16 mm C-mount lenses). Also M/CS mount adapter is available.
Screen
The small screen is a 3.5 inches TFT LCD capacitive touchscreen with a 480×320 resolution that supports up to 1920×1080. It is connected to the Raspberry Pi with a HDMI adapter for display, and to the GPIO port for the touchscreen function.
Lenses and Magnification
HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field.
The ocular is a magnifier meant to look at the intermediate image formed by the objective lens. Eye looks into eyepiece, thus it should produce a virtual image at infinity (the eye is relaxed). The object plane of the ocular is the image plane of object.
Total magnification: .
Aim: Wide magnification range: Samples with features from several centimeters to several micrometers can be imaged without changing the objective lens.
Particles with diameters between 1um and 10um. https://publiclab.org/notes/partsandcrafts/02-15-2018/2-attaching-your-raspberry-pi-camera-to-a-microscope-objective-lens
Microscope lenses: RMS Threaded stands for Royal Microscopy Society.
https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/
https://www.cambridgeincolour.com/tutorials/macro-lenses.htm
- Pimoroni Microscope Lens 60x-900x https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi
Motor
Stacking Photos
Aligning: http://hugin.sourceforge.net/
http://enblend.sourceforge.net/index.htm http://enblend.sourceforge.net/enfuse.doc/enfuse_4.1.xhtml/enfuse.xhtml#Focus-Stacks
https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/
https://www.cambridgeincolour.com/tutorials/focus-stacking.htm
Illumination
Reflected light
Transmitted light
Cross-section or contact angle
References
A lot of different ideas. Some are listed below
- https://www.raspberrypi.org/blog/a-low-cost-open-source-computer-assisted-microscope/
- https://microscopiproject.wordpress.com/
- https://www.raspberrypi.org/blog/raspberry-pi-high-quality-camera-powers-up-homemade-microscope/ Excellent images https://github.com/IBM/MicroscoPy
- https://hackaday.io/project/167996-pi-microscope
- https://www.instructables.com/ARPM-Another-raspberry-pi-microscope-made-from-Ple/
- https://publiclab.org/notes/MaggPi/04-08-2018/raspberry-pi-microscope-close-up-lens-system
- https://www.briandorey.com/post/raspberry-pi-high-quality-camera-on-the-microscope
- https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi
- https://openlabwaredotnet.files.wordpress.com/2015/11/main-v4.pdf
- https://magpi.raspberrypi.org/articles/openflexure-microscope
- https://diyprojects.io/motorized-microscope-hq-camera-raspberry-pi-python-html-interface/#.YRPo7YgzZEY
- https://publiclab.org/notes/partsandcrafts/11-26-2017/building-a-raspberry-pi-microscope
