Microscope and photo stacking - Revision history

Mol: /* Introduction */

2021-08-12T12:29:49Z

Introduction

← Older revision		Revision as of 15:29, 12 August 2021
Line 2:		Line 2:

	Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods.		Create a microscope with good magnification and automatically adjustable specimen board. Take automatically photos at different heights of the specimen board. Stack the photos using some methods.

			https://web.archive.org/web/20160917212243/http://photoblog.edu-perez.com/2009/01/greater-depth-field-macro.html

	== Theory ==		== Theory ==

2021-08-12T12:28:51Z

Stacking Photos

← Older revision		Revision as of 15:28, 12 August 2021
Line 43:		Line 43:
	Aligning: http://hugin.sourceforge.net/		Aligning: http://hugin.sourceforge.net/

	http://enblend.sourceforge.net/index.htm		http://enblend.sourceforge.net/index.htm http://enblend.sourceforge.net/enfuse.doc/enfuse_4.1.xhtml/enfuse.xhtml#Focus-Stacks

	https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/		https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/

2021-08-12T12:26:26Z

Stacking Photos

← Older revision		Revision as of 15:26, 12 August 2021
Line 41:		Line 41:
	=== Stacking Photos ===		=== Stacking Photos ===

			Aligning: http://hugin.sourceforge.net/

	http://enblend.sourceforge.net/index.htm		http://enblend.sourceforge.net/index.htm

2021-08-12T12:24:15Z

Stacking Photos

← Older revision		Revision as of 15:24, 12 August 2021
Line 41:		Line 41:
	=== Stacking Photos ===		=== Stacking Photos ===


			http://enblend.sourceforge.net/index.htm

	https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/		https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/

2021-08-12T12:23:04Z

Lenses and Magnification

← Older revision		Revision as of 15:23, 12 August 2021
Line 33:		Line 33:
	https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/		https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/

			https://www.cambridgeincolour.com/tutorials/macro-lenses.htm

	* Pimoroni Microscope Lens 60x-900x https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi		* Pimoroni Microscope Lens 60x-900x https://www.tomshardware.com/reviews/pimoroni-microscope-lens-for-raspberry-pi

2021-08-12T12:22:51Z

Stacking Photos

← Older revision		Revision as of 15:22, 12 August 2021
Line 39:		Line 39:

	=== Stacking Photos ===		=== Stacking Photos ===


			https://patdavid.net/2013/01/focus-stacking-macro-photos-enfuse/

			https://www.cambridgeincolour.com/tutorials/focus-stacking.htm

	=== Illumination ===		=== Illumination ===

2021-08-11T20:36:40Z

Lenses and Magnification

← Older revision		Revision as of 23:36, 11 August 2021
Line 16:		Line 16:
	HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field.		HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field.

	The ocular is a magnifier meant to look at the intermediate image formed by the objective lens. Eye looks into eyepiece, thus it should produce a virtual image at infinity (the eye is relaxed).		The ocular is a magnifier meant to look at the intermediate image formed by the objective lens. Eye looks into eyepiece, thus it should produce a virtual image at infinity (the eye is relaxed). The object plane of the ocular is the image plane of object.

			Total magnification: <math>M = M_\text{objective} \times M_\text{ocular}</math>.

	Aim: Wide magnification range: Samples with features from several centimeters to several micrometers can be imaged without changing the objective lens.		Aim: Wide magnification range: Samples with features from several centimeters to several micrometers can be imaged without changing the objective lens.

2021-08-11T20:34:42Z

Lenses and Magnification

← Older revision		Revision as of 23:34, 11 August 2021
Line 16:		Line 16:
	HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field.		HQ camera, lens, extension tube. 50mm C mount lens. The larger the extension ring, the shorter the depth of field.

			The ocular is a magnifier meant to look at the intermediate image formed by the objective lens. Eye looks into eyepiece, thus it should produce a virtual image at infinity (the eye is relaxed).

2021-08-11T15:46:07Z

Lenses and Magnification

← Older revision		Revision as of 18:46, 11 August 2021
Line 26:		Line 26:
	Microscope lenses: RMS Threaded stands for ''Royal Microscopy Society''.		Microscope lenses: RMS Threaded stands for ''Royal Microscopy Society''.

			https://www.seeedstudio.com/Microscope-Camera-300X-C-Mount-Lens-for-Raspberry-Pi-High-Quality-Camera-p-4627.html

	https://www.amscope.com/accessories/objective/100x-oil-achromatic-microscope-objective-for-compound-microscopes.html		https://www.amscope.com/accessories/objective/100x-oil-achromatic-microscope-objective-for-compound-microscopes.html

2021-08-11T15:45:35Z

Lenses and Magnification

← Older revision		Revision as of 18:45, 11 August 2021
Line 22:		Line 22:


			Particles with diameters between 1um and 10um. https://publiclab.org/notes/partsandcrafts/02-15-2018/2-attaching-your-raspberry-pi-camera-to-a-microscope-objective-lens

			Microscope lenses: RMS Threaded stands for ''Royal Microscopy Society''.


			https://www.amscope.com/accessories/objective/100x-oil-achromatic-microscope-objective-for-compound-microscopes.html

	https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/		https://www.nikonpassion.com/la-macrophotographie-guide-pratique-le-rapport-de-grandissement/